This project first began at Cincinnati Children's Hospital in the lab of Dr. Jeff Molkentin where Dr. Elrod established a high-throughput screening core. The goal of this project is to discover modulators of programmed necrosis with the hopes of finding new genetic targets to block cell death. The lab utilizes non-biased, genome-wide functional screening in cell-based assay systems. To aid in this discovery project different types of tools are utilized including shRNA lentiviral, cDNA, and small molecule libraries. Coupling these screening techniques with bioinformatics/systems biology analyses we have identified unique players and pathways in programmed necrosis and are investigating their mechanisms of action.